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KMID : 0613820120220020177
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Journal of Life Science
2012 Volume.22 No. 2 p.177 ~ p.183
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Isolation of Potato StACRE Gene and Its Function in Resistance against Bacterial Wilt Disease
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Park Sang-Ryeol
Cha Eun-Mi
Kim Tae-Hun
Han Se-Youn
Hwang Duk-Ju
Ahn Il-Pyung
Bae Shin-Chul
Cho Kwang-Soo
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Abstract
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Bacterial wilt (brown rot) caused by Ralstonia solanacearum (Rs) is one of the most devastating bacterial plant diseases in potatoes. To isolate bacterial wilt disease resistance-related genes from the potato, the StACRE (HM749652) gene was isolated and a sequenced search was performed using functional orthologs of Solanaceae from potatoes. StACRE is homologous to the tobacco NtACRE 132 protein and belongs to the ATL family involved in ubiquitination. To analyze the expression pattern of this gene, RT-PCR was performed with potato treated with salicylic acid (SA) and Rs (KACC 10722). StACRE was strongly induced 3 hours after treatment with SA and 12 hours after infection with Rs. To investigate its biological functions in the potato, we constructed a vector for overexpression in the potato by the Gateway system, and then generated transgenic potato plants. The gene expression of transgenic potato was analyzed by northern blot analysis. In the results of disease resistance assay in relation to bacterial wilt, StACRE overexpressed transgenic potato plants were shown to have more resistance than wild-type potato.
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KEYWORD
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Potato, StACRE, ubiquitination, disease resistance, bacterial wilt
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